Journal: Biotechnology and Bioengineering

Article Title: Segmented linear modeling of CHO fed‐batch culture and its application to large scale production

doi: 10.1002/bit.26214

Figure Lengend Snippet: Developed methodology to identify and characterize metabolic phases. Experimental data are first cleaned using the methodology presented in Figure 2 and additionally by removing data with a viability below 50% or a depletion of metabolites during a measurement interval. The number of metabolic phases during the cell culture process are determined by differentiating the smoothed (LOWESS) reaction rates of all metabolites with respect to the growth rate (dR/dµ). Recursive partitioning is then applied on those derivatives to get a vector of possible metabolic phase breakpoints. Hierarchical clustering is then applied on this vector of possible breakpoints to define the number of final metabolic phases (clusters). Knowing the number of metabolic phases, the segmented regression can then be calibrated on the calibration dataset for each metabolite and validated on the cross validation dataset of the 2 L bioreactor and also of the 2000 L bioreactor.

Article Snippet: As the derivative can amplify possible biological and analytical errors, the specific production rates were, preliminarily to deriving, smoothed as a function of the specific growth rate with the linear Locally Weighted Scatterplot Smoother (LOWESS) method (Cleveland, ) by using SAS software JMP 11 ©.

Techniques: Cell Culture, Plasmid Preparation, Biomarker Discovery

Journal: Scientific Reports

Article Title: Pharmacokinetic and pharmacodynamic similarity between SAR341402 insulin aspart and Japan-approved NovoRapid in healthy Japanese subjects

doi: 10.1038/s41598-021-02410-z

Figure Lengend Snippet: Linear arithmetic mean insulin aspart plasma time profiles ( A ), mean smoothed LOESS fits of body weight standardized GIR versus time profiles (GIR values less than 0 at baseline are results of the LOESS smoothing) ( B ). The horizontal line in ( A ) is the lower level of quantification (100 pg/mL). GIR glucose infusion rate, LOESS locally weighted scatterplot smoothing.

Article Snippet: Individual GIR values in each treatment group were standardized for body weight and subjected to a smoothing procedure using a locally weighted scatterplot smoothing (LOESS) function (SAS, PROC LOESS, factor 0.06).

Techniques: Clinical Proteomics